Research Papers On Cell Lines
Papers Based on Misidentified Cell Lines Top 32,000 | The Scientist ...
WIKIMEDIA, KAIBARA87Cell line misidentification is rampant throughout
biomedical research, and a new analysis quantifies its impact on the scientific
Research Papers On Cell Lines
An analysis of contaminated literature finds that tens of thousands of papers used cell lines of questionable originsand these were in turn cited by hundreds of thousands of other papers. The end result of cross-contamination is a cell line that no longer corresponds to the original, authentic culture. Up to now, only a few leading research laboratories and scientific journals are taking measures to change the dreadful situation of the continued use of wrongly denoted cell lines and, thereby, take responsibility for the publication of high-quality controlled data.
If the documents were not valid, the paper was returned to the authors (unsubmitted, which here means editorial consideration was paused). Cell banks and research groups share their datasets and publish on this topic (e. Cell lines are considered to represent cell functions in a given tissue of an organism and, therefore, have become invaluable tools for biomedical research.
Cell lines are used in life science research worldwide as biological surrogates. To maintain the role of biological surrogates, cell lines require constant quality control and assurance of their identity throughout their lifetime in vitro. It is possible that some authors who were nonnative speakers of english didnt understand our requirements correctly or had problems uploading a certificate in english.
Postgenomic research highlights continuously more detailed genetic and epigenetic differences between tumor entities originating from the same organ and even within the heterogeneous populations of a single tumor. All are now known to be misidentified, with the contaminating cell line arising from a different tissuefor example, gartler demonstrated that hep-2 is actually hela, arising from a female with cervical carcinoma usage of five misidentified cell lines in the scientific literature as shown by pubmed searches for cell line name and incorrect tissue identity. Another discussion point concerned limiting cell line documentation to a maximum of four years.
At submission, each paper was checked, and in order to monitor the history of the papers, we compiled and maintained a list in excel of those manuscripts with cell line problems at submission. Journals that include instructions or requirements for cell line authentication in their author guidelines , authors have continued to submit work that used misidentified cell lines. Authors only used further-modified cells or claimed that a cell line experiment is not really the main focus of the paper.
However, despite the fantastic work of individual reviewers, in general, the peer review process has mostly failed to detect and monitor the use of falsely designated cell lines. Isolation of pancreatic cancer cells from a patient-derived xenograft model allows for practical expansion and preserved heterogeneity in culture wrigley jd, mccall ej, bannaghan cl, liggins l, kendrick c, griffen a, et al. Common excuses are that this would be the task of reviewers, scientific societies, and funding organizations. In 2011, approximately 13 of papers received at the journal were unsubmitted for cell line issues and the authors were asked to upload the appropriate documents. Acceptance rate for all submissions and for papers previously unsubmitted for cell line issues, by continent in 20112013.
The need for a worldwide consensus for cell line authentication ...
17 Apr 2017 ... Cell lines are used in life science research worldwide as biological .... authors
have continued to submit work that used misidentified cell lines.
Research Papers On Cell Lines
Guidelines for the use of cell lines in biomedical research - NCBI - NIH
12 Aug 2014 ... Some journals insist on cell lines being made available as a condition of
publication, so that other laboratories can repeat the work.
Research Papers On Cell Lines
Postgenomic research highlights continuously more detailed genetic and epigenetic differences between tumor entities originating from the same organ and even within the heterogeneous populations of a single tumor. Papers are monitored regarding the following date of submission, title, author, country, resubmission or withdrawal, and final decision.
Author instructions rewritten to reflect new stipulation regarding cell lines. New testing methods became available, with john masters leading an initiative to assess the technique of short tandem repeat (str) profiling for cell line authentication.
20 Oct 2017. Unfortunately, the publishing community basically did not respond to this call to action.
We also compared the acceptance rate for the papers previously unsubmitted with that for all papers received from 2011 to 2013 ( ). Dna databases of str profiles from human cell lines are publicly available.
Cell culture - Latest research and news | Nature
International cell line authentication committee as not having authenticated stock, meaning they are likely mislabeled. The certificate provided does not fulfill the requirements (i. This trend was relatively stable with time, as shown for asia from 2011 to 2013 ( didnt lose papers worthy of acceptance on the contrary, unsubmitting some papers seemed to introduce another element in our triage towards better submissions, and authors who wanted to publish in our journal were mainly not discouraged by such a requirement. Other scientists with fewer resources, as in the case of developing countries, couldnt easily perform the necessary authentication, thus risking their chance of publication because of the new requirements. Thus, in the future, even more care has to be taken in selecting the correct cell lineincluding constant quality controlas well as in carefully interpreting the derived data.
As we outlined in an editorial recently published in the journal we are aiming to apply similar standards to address the problem of antibody specificity and for describing the animals used in research in better detail. An additional positive outcome was that our journal received a considerable amount of publicity, including reports in more widely read journals and magazines such as as well as in the national press in germany. The same amount of time per paper was needed again to log in the paper and update the excel file if the paper was resubmitted. Ultimately, we decided that the most effective way of handling the matter would be to establish a consortium of journals requiring proof of the authenticity of cells at manuscript submission. Cell line authentication represents one of the quality control measures at our journal, and for the future, we will aim to further refine our policies here to include, for example, requiring a statement in the paper itself, extending the requirement to murine lines, and advertising to a broader readership that cell lines used in papers published in our journal have been authenticated.
Author instructions rewritten to reflect new stipulation regarding cell lines. Str data for proof, or sent only raw data without a written statement of control and percentage of match. The standard and associated publications have reignited the warning to the scientific community that proper mandatory regulations for authentication need to be in place. The history of implementing such rules by the exemplifies the difficulties encountered when installing mandatory quality measures in life sciences. Ultimately, the hardest part of implementing the policy of requiring cell lines to be authenticated was taking the plunge and running the risk of not knowing how it would affect submissions, reputation, and workload for the editors and the editorial office. Especially at the beginning, many researchers were reluctant to check their own cell lines (see ). The american type culture collection (atcc) standard development organization established a working group of international experts to develop a written standard for human cell line authentication. A small number of scientific journalsthe being at the forefrontand funding agencies have recently moved forward to ask for obligatory cell line authentication data. Considerable administrative time and cost needed to be invested as a consequence we estimated approximately 240 hours of additional work over this three-year period to process papers that used cell lines (i. Many of the major cell banks took the initiative to inform their clients and withdraw false cell lines from their catalogues.Cell culture is a method for growing or maintaining cells in vitro under controlled
conditions. ... from tissues and have limited lifespan, whereas cell lines refer to
immortalized cells that can be cultured indefinitely. ... Scientific Reports 8, 16363.
Cancer Cell Lines for Drug Discovery and ... - Cancer Research1 May 2014 ... 12) assayed 130 drug responses in 639 human cancer lines. Although both
these studies used significant numbers of cell lines overall, the ...
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As a simple aid, we recommended that authors first check the published list of known cross-contaminated cell lines and then perform authentication. Indeed, worldwide, between 14 and 46 of the most commonly used cell lines are believed to be incorrectly designated. In contrast to the administrative times, which in the meantime have evened out with experience, these editorial times still apply and still vary according to quality of documentation and number of cell lines used. The german cancer research center has a mandatory requirement that all manuscripts to be submitted must authenticate the cell lines used in the experiments prior to submission. Looking at search results from these five cell lines, it is obvious that overall usage is steadily increasing Buy now Research Papers On Cell Lines
An even more disastrous feature is the cross-contamination that frequently occurs when cells from another culture are introduced by accident, resulting in replacement by a contaminant that has been propagated in the same laboratory or biosafety cabinet and is better adapted and faster growing than the original, authentic culture. In addition to cross-contamination, cell line misidentification can arise from incorrect labeling at the source. Misidentification of cell lines can happen in every laboratory, and without constant and vigorous control of authenticity, it often remains undetected. Pleasance ed, stephens pj, omeara s, mcbride dj, meynert a, jones d, et al. Thus, in the future, even more care has to be taken in selecting the correct cell lineincluding constant quality controlas well as in carefully interpreting the derived data Research Papers On Cell Lines Buy now
Ultimately, the hardest part of implementing the policy of requiring cell lines to be authenticated was taking the plunge and running the risk of not knowing how it would affect submissions, reputation, and workload for the editors and the editorial office. There is ample evidence that cross-contamination or phenotypic drift of cells in culture can generate irreproducible or misleading data. As outlined in nardones letter, many factors were weighed in our discussion, including the corruption of biomedical research in general as well as the matters of educating researchers and of compliance. Once the procedure was in place, we had to describe it in detail for our internal office manual. Thus, with easier authentication technologies and with more journals mandating cell line authentication before publication, acceptance and compliance with the new procedure have improved Buy Research Papers On Cell Lines at a discount
We verified whether cell lines were used and whether certificates were uploaded and fulfilled our criteria, which were as follows cell lines had been purchased from a reliable source within the past four years as per date on purchase order or invoice, cell lines were profiled within the past four years at a cell bank or reliable service provider and showed at least 80 identity, or cell lines were authenticated in the authors lab within the past four years with the profiling data provided and a form signed indicating that the results had been counterchecked against the standard database. Percentages of papers unsubmitted for cell line issues, by continent and by year. All are now known to be misidentified, with the contaminating cell line arising from a different tissuefor example, gartler demonstrated that hep-2 is actually hela, arising from a female with cervical carcinoma usage of five misidentified cell lines in the scientific literature as shown by pubmed searches for cell line name and incorrect tissue identity Buy Online Research Papers On Cell Lines
In the long run, this publicity may have furthered this important effort and also enhanced the it is important to ask whether these measures have addressed the problem of ongoing usage, however. As tumor cell lines often represent the first-line test objects for drug development, many biopharmaceutical companies have taken measures to check their cell lines regularly and employ cell line repositories, resulting in a low frequency of cell line misidentification we would make far more progress if cancer research labs and centers would follow a similar policy. The authors declare no cell lines used but on inspecting the manuscript, it appears that cell lines were used. In this initiative, the german collection of microorganisms and cell cultures (leibniz institut-dsmz) has been a pioneer and is still a major actor in the process Buy Research Papers On Cell Lines Online at a discount
We also compared the acceptance rate for the papers previously unsubmitted with that for all papers received from 2011 to 2013 ( ). Prominent journals that currently incorporate authentication testing into their author guidelines are listed in. To maintain the role of biological surrogates, cell lines require constant quality control and assurance of their identity throughout their lifetime in vitro. Cell lines are considered to represent cell functions in a given tissue of an organism and, therefore, have become invaluable tools for biomedical research. In 2011, approximately 13 of papers received at the journal were unsubmitted for cell line issues and the authors were asked to upload the appropriate documents Research Papers On Cell Lines For Sale
Papers unsubmitted for cell line issues and then resubmitted, by continent in 20112013. Unfortunately, the publishing community basically did not respond to this call to action. International society of managing and technical editors (ismte) 2012 in addition to the considerable time and cost involved in monitoring the process, these new requirements also had to be included in the author instructions, in the email template sent to the authors to ask for suitable certificates, and in the submission questions in the scholarone manuscript system. Implementing such a procedure at the journal was not without difficulties and required a series of administrative and editorial measures, as summarized in For Sale Research Papers On Cell Lines
In 2010, since no joint initiative with leading journals and granting organizations could be established, the and after a short pilot period during which authors were encouraged to authenticate their lines, we made it obligatory that authors submitting to the journal provide dna-based certification of the identity of the cell lines used in their work. We also needed to expand our procedures for logging in manuscripts to include checking whether cell lines had been used in a submitted manuscript and whether the proper documentation had been provided ( ). Manuscript managing system (scholarone) updated to include new questions for authors about whether or not they authenticated their cell lines Sale Research Papers On Cell Lines
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